Scientific internship: Deep-tissue super-resolution neuroimaging

Date posted December 12, 2019
Type Scientific internships

At the Advanced Research Center for Nanolithography (ARCNL) we carry out exciting fundamental physics at the highest possible level with relevance to key technologies in nanolithography. We contribute to the production of ever smarter and smaller electronics, while at the same time pushing the boundaries of our fundamental insight into the workings of nature.

For several centuries, far-field optical microscopy remains a key instrument in many scientific disciplines including physical, chemical and biomedical research. Unfortunately, biological tissues strongly scatter light, making high-resolution deep-tissue imaging impossible for traditional light field microscopy. We are creating a breakthrough method for in vivo bioimaging. In this project we will develop new endo-microscopy approaches for deep-tissue ultra-fast neuroimaging with the spatial resolution beyond the diffraction limit.

In collaboration with the LaserLaB Amsterdam and Center for Neurogenomics and Cognitive Research, VU, Amsterdam, super-resolution endomicroscopy will be applied to imaging the functional distribution of biomarkers in brain tissue. The new endoscopic method will allow to visualize subcellular structures, such as dendritic spines, and study neuropeptide transmission deep inside the intact brain. It will provide a new tool to investigate the role of neuropeptides at a functional level and their implication in neurodegenerative diseases.

Recommended articles for further reading on this subject:

  1. L. V. Amitonova and J. F. de Boer, “Compressive imaging through a multimode fiber,” Optics Letters 43, 5427 (2018).
  2. Y. Sych, M. Chernysheva, L. T. Sumanovski, and F. Helmchen, “High-density multi-fiber photometry for studying large-scale brain circuit dynamics,” Nature Methods 16, 553 (2019).
  3. L. V. Doronina-Amitonova, et al. Implantable fiber-optic interface for parallel multisite long-term optical dynamic brain interrogation in freely moving mice. Scientific Reports 3, 3265 (2013).

About the group

The Nanoscale Imaging and Metrology group is a new research group at ARCNL, headed by Dr. Lyuba Amitonova. Our ultimate goal is imaging beyond the limits. We are developing new label-free far-field imaging techniques with a spatial resolution beyond the Abbe (diffraction) limit and temporal resolution beyond the Nyquist limit. The work will be done at VU Amsterdam.

Qualifications

You have a Bachelor’s degree in physics or a related field and participate in a Master study. The internship must be a mandatory part of your curriculum. You have a nationality of an EU-member state and/or you are a student at a Netherlands University. You must be available for at least 6 months.

Terms of employment

At the start of the traineeship your trainee plan will be set out, in consultation with your ARCNL supervisor, including a small allowance.

Contact info

Dr. Lyuba Amitonova
Group leader Nanoscale Imaging and Metrology
E-mail: l.amitonova@arcnl.nl; l.amitonova@vu.nl
Phone: +31(0)20 851 7100

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Please annex your:
–  Resume;
–  List of followed courses plus grades.

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